Computational modeling and validation studies of 3-D structure of neuraminidase protein of H1N1 influenza A virus and subsequent in silico elucidation of piceid analogues as its potent inhibitors
Identifieur interne : 000903 ( Main/Exploration ); précédent : 000902; suivant : 000904Computational modeling and validation studies of 3-D structure of neuraminidase protein of H1N1 influenza A virus and subsequent in silico elucidation of piceid analogues as its potent inhibitors
Auteurs : Chhedi Lal Gupta [Inde] ; Salman Akhtar [Inde] ; Preeti Bajpaib [Inde] ; K. N. Kandpal [Inde] ; G. S. Desai [Inde] ; Ashok K. Tiwari [Inde]Source :
- EXCLI Journal [ 1611-2156 ] ; 2013.
Abstract
Emergence of the drug resistant variants of the Influenza A virus in the recent years has aroused a great need for the development of novel neuraminidase inhibitors for controlling the pandemic. The neuraminidase (NA) protein of the influenza virus has been the most potential target for the anti-influenza. However, in the absence of any experimental structure of the drug targeting NA protein of H1N1 influenza A virus as zanamivir and oseltamivir, the comprehensive study of the interaction of the drug molecules with the target protein has been missing. Hence in this study a computational 3-D structure of neuraminidase of H1N1 influenza A virus has been developed using homology modeling technique, and the same was validated for its reliability by ProSA web server in term of energy profile & Z scores and PROCHECK program followed by Ramachandran plot. Further, the developed 3-D model had been employed for docking studies with the class of compounds as Piceid and its analogs. In this context, two novel compounds (ChemBank ID 2110359 and 3075417) were found to be more potent inhibitors of neuraminidase than control drugs as zanamivir and oseltamivir in terms of their robust binding energies, strong inhibition constant (Ki) and better hydrogen bond interactions between the protein-ligand complex. The interaction of these compounds with NA protein has been significantly studied at the molecular level.
Url:
PubMed: 26417228
PubMed Central: 4556016
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000029
- to stream Pmc, to step Curation: 000029
- to stream Pmc, to step Checkpoint: 000637
- to stream Ncbi, to step Merge: 000C28
- to stream Ncbi, to step Curation: 000C28
- to stream Ncbi, to step Checkpoint: 000C28
- to stream Main, to step Merge: 000904
- to stream Main, to step Curation: 000903
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Computational modeling and validation studies of 3-D structure of neuraminidase protein of H1N1 influenza A virus and subsequent in silico elucidation of piceid analogues as its potent inhibitors</title><author><name sortKey="Gupta, Chhedi Lal" sort="Gupta, Chhedi Lal" uniqKey="Gupta C" first="Chhedi Lal" last="Gupta">Chhedi Lal Gupta</name><affiliation wicri:level="1"><nlm:aff id="A1">Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122</wicri:regionArea><wicri:noRegion>Izatnagar–243122</wicri:noRegion></affiliation></author><author><name sortKey="Akhtar, Salman" sort="Akhtar, Salman" uniqKey="Akhtar S" first="Salman" last="Akhtar">Salman Akhtar</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026</wicri:regionArea><wicri:noRegion>Lucknow-226026</wicri:noRegion></affiliation></author><author><name sortKey="Bajpaib, Preeti" sort="Bajpaib, Preeti" uniqKey="Bajpaib P" first="Preeti" last="Bajpaib">Preeti Bajpaib</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026</wicri:regionArea><wicri:noRegion>Lucknow-226026</wicri:noRegion></affiliation></author><author><name sortKey="Kandpal, K N" sort="Kandpal, K N" uniqKey="Kandpal K" first="K. N." last="Kandpal">K. N. Kandpal</name><affiliation wicri:level="1"><nlm:aff id="A1">Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122</wicri:regionArea><wicri:noRegion>Izatnagar–243122</wicri:noRegion></affiliation></author><author><name sortKey="Desai, G S" sort="Desai, G S" uniqKey="Desai G" first="G. S." last="Desai">G. S. Desai</name><affiliation wicri:level="1"><nlm:aff id="A3">Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122</wicri:regionArea><wicri:noRegion>Izatnagar-243122</wicri:noRegion></affiliation></author><author><name sortKey="Tiwari, Ashok K" sort="Tiwari, Ashok K" uniqKey="Tiwari A" first="Ashok K." last="Tiwari">Ashok K. Tiwari</name><affiliation wicri:level="1"><nlm:aff id="A1">Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122</wicri:regionArea><wicri:noRegion>Izatnagar–243122</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26417228</idno><idno type="pmc">4556016</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556016</idno><idno type="RBID">PMC:4556016</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000029</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000029</idno><idno type="wicri:Area/Pmc/Curation">000029</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000029</idno><idno type="wicri:Area/Pmc/Checkpoint">000637</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000637</idno><idno type="wicri:Area/Ncbi/Merge">000C28</idno><idno type="wicri:Area/Ncbi/Curation">000C28</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C28</idno><idno type="wicri:Area/Main/Merge">000904</idno><idno type="wicri:Area/Main/Curation">000903</idno><idno type="wicri:Area/Main/Exploration">000903</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Computational modeling and validation studies of 3-D structure of neuraminidase protein of H1N1 influenza A virus and subsequent in silico elucidation of piceid analogues as its potent inhibitors</title><author><name sortKey="Gupta, Chhedi Lal" sort="Gupta, Chhedi Lal" uniqKey="Gupta C" first="Chhedi Lal" last="Gupta">Chhedi Lal Gupta</name><affiliation wicri:level="1"><nlm:aff id="A1">Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122</wicri:regionArea><wicri:noRegion>Izatnagar–243122</wicri:noRegion></affiliation></author><author><name sortKey="Akhtar, Salman" sort="Akhtar, Salman" uniqKey="Akhtar S" first="Salman" last="Akhtar">Salman Akhtar</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026</wicri:regionArea><wicri:noRegion>Lucknow-226026</wicri:noRegion></affiliation></author><author><name sortKey="Bajpaib, Preeti" sort="Bajpaib, Preeti" uniqKey="Bajpaib P" first="Preeti" last="Bajpaib">Preeti Bajpaib</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Microbiology and Bioinformatics, Integral University, Lucknow-226026</wicri:regionArea><wicri:noRegion>Lucknow-226026</wicri:noRegion></affiliation></author><author><name sortKey="Kandpal, K N" sort="Kandpal, K N" uniqKey="Kandpal K" first="K. N." last="Kandpal">K. N. Kandpal</name><affiliation wicri:level="1"><nlm:aff id="A1">Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122</wicri:regionArea><wicri:noRegion>Izatnagar–243122</wicri:noRegion></affiliation></author><author><name sortKey="Desai, G S" sort="Desai, G S" uniqKey="Desai G" first="G. S." last="Desai">G. S. Desai</name><affiliation wicri:level="1"><nlm:aff id="A3">Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122</wicri:regionArea><wicri:noRegion>Izatnagar-243122</wicri:noRegion></affiliation></author><author><name sortKey="Tiwari, Ashok K" sort="Tiwari, Ashok K" uniqKey="Tiwari A" first="Ashok K." last="Tiwari">Ashok K. Tiwari</name><affiliation wicri:level="1"><nlm:aff id="A1">Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122, India</nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Bioinformatics Centre, Indian Veterinary Research Institute, Izatnagar–243122</wicri:regionArea><wicri:noRegion>Izatnagar–243122</wicri:noRegion></affiliation></author></analytic><series><title level="j">EXCLI Journal</title><idno type="eISSN">1611-2156</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Emergence of the drug resistant variants of the Influenza A virus in the recent years has aroused a great need for the development of novel neuraminidase inhibitors for controlling the pandemic. The neuraminidase (NA) protein of the influenza virus has been the most potential target for the anti-influenza. However, in the absence of any experimental structure of the drug targeting NA protein of H1N1 influenza A virus as zanamivir and oseltamivir, the comprehensive study of the interaction of the drug molecules with the target protein has been missing. Hence in this study a computational 3-D structure of neuraminidase of H1N1 influenza A virus has been developed using homology modeling technique, and the same was validated for its reliability by ProSA web server in term of energy profile & Z scores and PROCHECK program followed by Ramachandran plot. Further, the developed 3-D model had been employed for docking studies with the class of compounds as Piceid and its analogs. In this context, two novel compounds (ChemBank ID 2110359 and 3075417) were found to be more potent inhibitors of neuraminidase than control drugs as zanamivir and oseltamivir in terms of their robust binding energies, strong inhibition constant (Ki) and better hydrogen bond interactions between the protein-ligand complex. The interaction of these compounds with NA protein has been significantly studied at the molecular level.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Altschul, Sf" uniqKey="Altschul S">SF Altschul</name></author><author><name sortKey="Madden, Tl" uniqKey="Madden T">TL Madden</name></author><author><name sortKey="Schaffer" uniqKey="Schaffer">Schaffer</name></author><author><name sortKey="Aa" uniqKey="Aa">AA</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z Zhang</name></author><author><name sortKey="Miller, W" uniqKey="Miller W">W Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anh Nguyen, Tn" uniqKey="Anh Nguyen T">TN Anh Nguyen</name></author><author><name sortKey="Dao, Tt" uniqKey="Dao T">TT Dao</name></author><author><name sortKey="Tung, Bt" uniqKey="Tung B">BT Tung</name></author><author><name sortKey="Choi, H" uniqKey="Choi H">H Choi</name></author><author><name sortKey="Kim, E" uniqKey="Kim E">E Kim</name></author><author><name sortKey="Park, J" uniqKey="Park J">J Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buch, I" uniqKey="Buch I">I Buch</name></author><author><name sortKey="Giorgino, T" uniqKey="Giorgino T">T Giorgino</name></author><author><name sortKey="De Fabritiis, G" uniqKey="De Fabritiis G">G De Fabritiis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, Qs" uniqKey="Du Q">QS Du</name></author><author><name sortKey="Wang, Sq" uniqKey="Wang S">SQ Wang</name></author><author><name sortKey="Huang, Rb" uniqKey="Huang R">RB Huang</name></author><author><name sortKey="Chou, Kc" uniqKey="Chou K">KC Chou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ho, Ht" uniqKey="Ho H">HT Ho</name></author><author><name sortKey="Hurt, Ac" uniqKey="Hurt A">AC Hurt</name></author><author><name sortKey="Mosse, J" uniqKey="Mosse J">J Mosse</name></author><author><name sortKey="Barr, I" uniqKey="Barr I">I Barr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huey, R" uniqKey="Huey R">R Huey</name></author><author><name sortKey="Morris, Gm" uniqKey="Morris G">GM Morris</name></author><author><name sortKey="Olson, Aj" uniqKey="Olson A">AJ Olson</name></author><author><name sortKey="Goodsell, Ds" uniqKey="Goodsell D">DS Goodsell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lam, Ks" uniqKey="Lam K">KS Lam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laskowski, Ra" uniqKey="Laskowski R">RA Laskowski</name></author><author><name sortKey="Macarthur, Mw" uniqKey="Macarthur M">MW MacArthur</name></author><author><name sortKey="Moss, Ds" uniqKey="Moss D">DS Moss</name></author><author><name sortKey="Thornton, Jm" uniqKey="Thornton J">JM Thornton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lew, W" uniqKey="Lew W">W Lew</name></author><author><name sortKey="Chen, X" uniqKey="Chen X">X Chen</name></author><author><name sortKey="Kim, Cu" uniqKey="Kim C">CU Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liang, S" uniqKey="Liang S">S Liang</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Hsu, Wl" uniqKey="Hsu W">WL Hsu</name></author><author><name sortKey="Pilcher, Mn" uniqKey="Pilcher M">MN Pilcher</name></author><author><name sortKey="Uversky, V" uniqKey="Uversky V">V Uversky</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mackerell, Ad" uniqKey="Mackerell A">AD MacKerell</name></author><author><name sortKey="Jr" uniqKey="Jr">Jr</name></author><author><name sortKey="Bashford, D" uniqKey="Bashford D">D Bashford</name></author><author><name sortKey="Bellott, M" uniqKey="Bellott M">M Bellott</name></author><author><name sortKey="Dunbrack, Rl" uniqKey="Dunbrack R">RL Dunbrack</name></author><author><name sortKey="Jr" uniqKey="Jr">Jr</name></author><author><name sortKey="Evanseck, Jd" uniqKey="Evanseck J">JD Evanseck</name></author><author><name sortKey="Field, Mj" uniqKey="Field M">MJ Field</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mckimm Reschkin, Jl" uniqKey="Mckimm Reschkin J">JL McKimm–Breschkin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morris, Gm" uniqKey="Morris G">GM Morris</name></author><author><name sortKey="Goodsell, Ds" uniqKey="Goodsell D">DS Goodsell</name></author><author><name sortKey="Halliday, Rs" uniqKey="Halliday R">RS Halliday</name></author><author><name sortKey="Huey, R" uniqKey="Huey R">R Huey</name></author><author><name sortKey="Hart, We" uniqKey="Hart W">WE Hart</name></author><author><name sortKey="Belew, Rk" uniqKey="Belew R">RK Belew</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Newman, Dj" uniqKey="Newman D">DJ Newman</name></author><author><name sortKey="Cragg, Gm" uniqKey="Cragg G">GM Cragg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pettersen, Ef" uniqKey="Pettersen E">EF Pettersen</name></author><author><name sortKey="Goddard, Td" uniqKey="Goddard T">TD Goddard</name></author><author><name sortKey="Huang, Cc" uniqKey="Huang C">CC Huang</name></author><author><name sortKey="Couch, Gs" uniqKey="Couch G">GS Couch</name></author><author><name sortKey="Greenblatt, Dm" uniqKey="Greenblatt D">DM Greenblatt</name></author><author><name sortKey="Meng, Ec" uniqKey="Meng E">EC Meng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sakudo, A" uniqKey="Sakudo A">A Sakudo</name></author><author><name sortKey="Baba, K" uniqKey="Baba K">K Baba</name></author><author><name sortKey="Tsukamoto, M" uniqKey="Tsukamoto M">M Tsukamoto</name></author><author><name sortKey="Sugimoto, A" uniqKey="Sugimoto A">A Sugimoto</name></author><author><name sortKey="Okada, T" uniqKey="Okada T">T Okada</name></author><author><name sortKey="Kobayashi, T" uniqKey="Kobayashi T">T Kobayashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sali, A" uniqKey="Sali A">A Sali</name></author><author><name sortKey="Blundell, Tl" uniqKey="Blundell T">TL Blundell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sali, A" uniqKey="Sali A">A Sali</name></author><author><name sortKey="Overington, Jp" uniqKey="Overington J">JP Overington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sarkar, M" uniqKey="Sarkar M">M Sarkar</name></author><author><name sortKey="Agrawal, As" uniqKey="Agrawal A">AS Agrawal</name></author><author><name sortKey="Sharma Dey, R" uniqKey="Sharma Dey R">R Sharma Dey</name></author><author><name sortKey="Chattopadhyay, S" uniqKey="Chattopadhyay S">S Chattopadhyay</name></author><author><name sortKey="Mullick, R" uniqKey="Mullick R">R Mullick</name></author><author><name sortKey="De, P" uniqKey="De P">P De</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Solis, Fj" uniqKey="Solis F">FJ Solis</name></author><author><name sortKey="Wets, Jb" uniqKey="Wets J">JB Wets</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steiner, T" uniqKey="Steiner T">T Steiner</name></author><author><name sortKey="Koellner, G" uniqKey="Koellner G">G Koellner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, Jd" uniqKey="Thompson J">JD Thompson</name></author><author><name sortKey="Higgins, Dg" uniqKey="Higgins D">DG Higgins</name></author><author><name sortKey="Gibson, Tj" uniqKey="Gibson T">TJ Gibson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weiss, Ms" uniqKey="Weiss M">MS Weiss</name></author><author><name sortKey="Brandl, M" uniqKey="Brandl M">M Brandl</name></author><author><name sortKey="Suhnel, J" uniqKey="Suhnel J">J Sühnel</name></author><author><name sortKey="Pal, D" uniqKey="Pal D">D Pal</name></author><author><name sortKey="Hilgenfeld, R" uniqKey="Hilgenfeld R">R Hilgenfeld</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wiederstein, M" uniqKey="Wiederstein M">M Wiederstein</name></author><author><name sortKey="Sippl, Mj" uniqKey="Sippl M">MJ Sippl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author><author><name sortKey="Zhu, X" uniqKey="Zhu X">X Zhu</name></author><author><name sortKey="Dwek, Ra" uniqKey="Dwek R">RA Dwek</name></author><author><name sortKey="Stevens, J" uniqKey="Stevens J">J Stevens</name></author><author><name sortKey="Wilson, Ia" uniqKey="Wilson I">IA Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yadav, Bs" uniqKey="Yadav B">BS Yadav</name></author><author><name sortKey="Tripathi, V" uniqKey="Tripathi V">V Tripathi</name></author><author><name sortKey="Kumar, A" uniqKey="Kumar A">A Kumar</name></author><author><name sortKey="Khan, Mf" uniqKey="Khan M">MF Khan</name></author><author><name sortKey="Kumar, A" uniqKey="Kumar A">A Kumar</name></author><author><name sortKey="Sharma, B" uniqKey="Sharma B">B Sharma</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Gupta, Chhedi Lal" sort="Gupta, Chhedi Lal" uniqKey="Gupta C" first="Chhedi Lal" last="Gupta">Chhedi Lal Gupta</name></noRegion><name sortKey="Akhtar, Salman" sort="Akhtar, Salman" uniqKey="Akhtar S" first="Salman" last="Akhtar">Salman Akhtar</name><name sortKey="Bajpaib, Preeti" sort="Bajpaib, Preeti" uniqKey="Bajpaib P" first="Preeti" last="Bajpaib">Preeti Bajpaib</name><name sortKey="Desai, G S" sort="Desai, G S" uniqKey="Desai G" first="G. S." last="Desai">G. S. Desai</name><name sortKey="Kandpal, K N" sort="Kandpal, K N" uniqKey="Kandpal K" first="K. N." last="Kandpal">K. N. Kandpal</name><name sortKey="Tiwari, Ashok K" sort="Tiwari, Ashok K" uniqKey="Tiwari A" first="Ashok K." last="Tiwari">Ashok K. Tiwari</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000903 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000903 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:4556016
|texte= Computational modeling and validation studies of 3-D structure of neuraminidase protein of H1N1 influenza A virus and subsequent in silico elucidation of piceid analogues as its potent inhibitors
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26417228" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |